New publication:Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

14 July 2020

Photo: UHH/CHyN/AG Blick/Harberts

We successfully published work by our BioMat-team around Cornelius and Jann of neurite guiding and caging 2.5D structured substrates in RSC Nanoscale Advances https://doi.org/10.1039/D0NA00549E.

The publication is published under Open-Access license.

Abstract:

Directed guidance of neurites is a pre-requisite for tailor-made designs of interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, and ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques for a cost- and time-effective fabrication of modulated surfaces. In this work, neurite outgrowth of murine cerebellar neurons on 2.5D pathways produced with these methods is studied. Structures of micron-sized steps and grooves serve as cell culture platforms. The effects of contact guidance through topography and chemical guidance through selective poly-D-lysine coating on these platforms are analyzed. As a consequence, the herein presented fabrication approach can be utilized to cultivate and to study low-density neuronal networks in 2.5D configuration with a high degree of order.